Clinico-pathological Evaluation of Hepatoprotective Effect of Dandelion and Ginkgo-biloba Extract against Thioacetamide Induced Liver Damage

Abstract

This study was carried outto investigatethe ameliorative effect of both of Dandelion and Ginko.biloba extract on liver toxicities induced by thioacetamide (TAA) in male albino rats.Sixty male albino rats were divided into sixgroups (10 rats pereach).Group 1 (Negative control): which received 1 ml of saline orally and 1ml of saline intra-peritoneal (I/P),Group 2: received TAA for induction of hepatic injuryGroup 3:received TAA+ ginkgo biloba extract (50 mg/ b.wt.),Group 4: received TAA+ ginkgo biloba extract (100 mg/ kg b.wt.).Group 5: which received TAA + dandelion extract (250 mg/ kg b.wt).Group 6: which received TAA+ dandelion extract (500 mg/ kg b.wt.).TAA was administrated by I/P route at dose level of 250mg/kg b.wt , extracts were administrated orally, they were administrated 3 times/week for 4 weeks. The obtained results revealed that intoxication with TAAshowed a significant elevation in serum liver enzymes activity, total bilirubinplussignificant decrease in serum levels of total proteins andalbumin, significant increase in total cholesterol and triglycerides.At the same time ,intoxication with TAA exhibited severe oxidative damged stress ,represented by a significant increase in hepatic level of malondialdehyde (MDA)as well asa significant decrease in reduced glutathione (GSH).The characteristic pathological changes were represented by ,(TAA),severe degenerative andnecrotic alterationsin the hepatocytes. The treatment with G.biloba and D- in different treateddosesattenuatedTAA-induced,serum biochemical alterations, oxidative stressand histopathological changes in liver.