Clinico-immunological justification of the use of a new dental ointment developed on the basis of a long-acting beekeeping product for topical treatment of inflammatory parodontal diseases

Abstract

Relevance of the research topic. The composition and technology for the manufacture of dental ointment based on antimicrobial metronidazole, anesthetic anesthesin and an immunomodula-tor are substantiated and developed - for the frst time a biological product of the beekeeping product of a large wax moth larvae extract.Puprose – the goal is a clinical and immunological rationale for the use of dental ointment developed composition based on the product of beekeeping for the treatment of inflammatory periodontal diseases. Materials and methods. Studies have been conducted in patients with inflammatory periodontal disease (CDL), aged from 20 to 70 years. Group I control (n = 25), who had no dental pathology and practically healthy at the time of examination, group II (n = 204) received basic thera-py, group III (n = 120) used a developed dental ointment based on ( Galleria mellonela). Evaluation of the effectiveness of treatment was carried out according to clinical and laboratory parameters. Analysis of the hygienic and clinical state of periodontal tissues was taken into account by the Green-Vermillion, Russel, PMA indices in the Parma modifcation. The state of local immunity was investi-gated according to the indicators of the leukocyte migration test, the quantitative and qualitative con-tent of spontaneously released mixed saliva, the content of total protein in it, secretory immunoglobu-lin A, lysozyme.Results. The effectiveness of the developed dental ointment is confrmed by the positive dy-namics of clinical indicators in comparison with the basic therapy in 72.3% of cases. The number of sessions per course of treatment for gingivitis and mild periodontitis was reduced to 3, moderate –5–6, and severe to 8 sessions. Indices (GI, PI, PMA) decreased by 6.7; 2.6; 5.1 times, respectively, in comparison with the group before treatment and in 3; 2; 2.6 times, respectively, compared with base-line therapy. Local immunity revealed: an increase in the production of mixed saliva in comparison with baseline data by 2.6 times, its recovery and an increase of 1 ml above the control level (p> 0.05), an increase in protein level (p <0.05), lysozyme concentration and sIgA exceeded the level before the treatment and after the baseline therapy, however, the indices did not recover to the level in the con-trol.Summaru. Inclusion of dental ointment on the basis of Galleria mellonell into the complex therapy of VZP is a means of correcting violations of local immunity and is a promising new dosage form in practical periodontology