Abstract
BackgroundCirculating tumor cells (CTCs) are the dominant factor leading to tumor metastasis. This study aims to investigate the effect of disparate sources of CTCs on the treatment and prognosis of patients with advanced tumors by analyzing the number and gene mutations change of CTCs in arterial and venous blood in patients with advanced tumors.ResultsA CTCs sorting system was constructed based on Vimentin-immunolipid magnetic balls (Vi-IMB) and EpCAM immunolipid magnetic balls (Ep-IMB). Results showed that the prepared Ep-IMB and Vi-IMB had lower cytotoxicity, better specificity and sensitivity. The number of arterial CTCs was higher than that of venous CTCs, with a statistically significant difference (P < 0.05). Moreover, the prognosis of the low positive group of total CTCs in arterial blood and venous blood was higher than that of the high positive group, with a statistical significance (P < 0.05). The genetic testing results showed that the targeted drug gene mutations in tissues, arterial CTCs and venous CTCs showed a complementary trend, indicating that there was heterogeneity among different tumor samples.ConclusionsCTCs in blood can be efficiently captured by the CTCs sorting system based on Vi-LMB/Ep-LMB, and CTCs detection in arterial blood can be utilized to more accurately evaluate the prognosis and predict postoperative progress. It is further confirmed that tumor samples from disparate sources are heterogeneous, providing a reference basis for gene mutation detection before clinical targeted drug treatment, and the detection of CTCs in arterial blood has more potential clinical application value.Trial registration: The Ethics Committee of Putuo Hospital, PTEC-A-2019-18-1. Registered 24 September 2019.Graphic abstract
Highlights
Circulating tumor cells (CTCs) are the dominant factor leading to tumor metastasis
In the Fourier transform infrared (FT-IR) spectra of EpCAM modified lipid magnetic balls (Ep-lipid magnetic ball (LMB)) and Vi-LMB, new peaks were found at about 2840–2930 cm−1 due to the long carbon chain and methyl groups on the quaternary ammonium salt, indicating the presence of GHDC on both Ep-LMB and Vi-LMB
GHDC was conjugated with epithelial cell adhesion molecule (EpCAM) and Vimentin respectively, indirectly indicating that EpCAM and vimentin antibodies have been conjugated on the surface of Ep-LMB and Vi-LMB
Summary
Circulating tumor cells (CTCs) are the dominant factor leading to tumor metastasis. This study aims to investigate the effect of disparate sources of CTCs on the treatment and prognosis of patients with advanced tumors by analyzing the number and gene mutations change of CTCs in arterial and venous blood in patients with advanced tumors. Tumor recurrence and metastasis are still the leading causes of. Recent studies have demonstrated that even in the early stage of cancer, tumor cells can enter the blood circulation system directly or indirectly via the lymphatic pathway, and colonize in distant tissues or organs and eventually proliferate to form metastasis. The detection of CTCs in peripheral blood of patients with tumor is of great clinical significance in early diagnosis, disease progression and prognosis judgment, and real-time monitoring of curative effects of breast cancer, colorectal cancer, lung cancer, gastric cancer, liver cancer, pancreatic cancer, etc. The detection of CTCs in peripheral blood of patients with tumor is of great clinical significance in early diagnosis, disease progression and prognosis judgment, and real-time monitoring of curative effects of breast cancer, colorectal cancer, lung cancer, gastric cancer, liver cancer, pancreatic cancer, etc. [7,8,9,10,11,12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
