Clinical significance of nine-item combined reagent for detection of pathogens in various respiratory tract infection

Abstract

Objective To introduce a rapid approach for the diagnosis of pathogens in various respiratory tract infection.Methods The sera were collected from 1318 patients with respiratory tract infection and were subjected to simultaneous detection of immunoglobin M of nine major pathogens,including Legionella pneumophila (LP), Mycoplasma pneumonia ( MP), Coxiella burneti ( COX ), Chlamydia pneumoniae ( CP), adenovirus ( ADV), respiratory syncytial virus ( RSV), influenza A virus(IFA), influenza B virus B (IFB) and parainfluenza virus (PIVS) type 1,2 and 3,via indirect immunofluorescence assay. Results Infection of atypical pathogen,predominantly MP,followed by RSV,IFB and IFA,accounted for 33.3% (439/1318).Combined pathogen infection,to which children were predisposed,amounted to 15.7% (207/1318).Conclusions Indirect immunofluorescence assay using nine-item combined reagent can be applied extensively as a result of rapid detection,simplified processing,high accuracy and low cost.This may be helpful upon diagnosis in a timely manner, allowing for further popularization among various hospitals. Key words: Respiratory tract infection; Specific pathogen - free organisms; Fluorescent antibody technique , indirect; Antibodies