Abstract
BackgroundMicroRNAs (miRNAs) play important roles in the development and progression of sepsis. This study investigated the clinical value of miR-19b-3p in sepsis patients, and explored its role in regulating inflammatory responses in HUVECs cells.Methods103 patients with sepsis and 98 healthy individuals were recruited. qRT-PCR was used for the measurement of miR-19b-3p level. Cell viability was evaluated using CCK-8. The protein levels of TNF-α and IL-6 were measured using ELISA. Receiver operating characteristic (ROC) curve and logistic regression analysis were constructed to evaluate the diagnostic and prognostic values of miR-19b-3p in sepsis patients.ResultsMiR-19b-3p level was significantly reduced in the serum from patients with sepsis compared with healthy controls (P < 0.001). Sepsis patients in the survival group had significantly high miR-19b-3p levels compared with the non-survival group (P < 0.001). MiR-19b-3p was of a good value in predicting sepsis risk, and was an independent prognostic factor for 28-day survival in sepsis patients (OR = 3.226, 95% CI 1.076–9.670, P = 0.037). MiR-19b-3p level was negatively associated with serum levels of IL-6 (r = − 0.852, P < 0.001) and TNF-α (r = − 0.761, P < 0.001). Overexpression of miR-19b-3p alleviated LPS-induced inflammatory response of HUVECs, which was reflected by the decrease of the levels of IL-6 and TNF-α induced by LPS treatment (P < 0.001).ConclusionMiR-19b-3p might be a potential biomarker for the early diagnosis and prognosis of sepsis patients. Overexpression of miR-19b-3p alleviated sepsis-induced inflammatory responses.
Highlights
MicroRNAs play important roles in the development and progression of sepsis
A cohort of sepsis patients were recruited, and miR-19b-3p expression was determined to be downregulated in the serum of sepsis patients, we further explored its clinical values in sepsis patients
According to the qRTPCR results, we observed that miR-19b-3p level was significantly reduced in the serum from patients with sepsis compared with healthy controls (Fig. 1a, P < 0.001)
Summary
MicroRNAs (miRNAs) play important roles in the development and progression of sepsis. This study investigated the clinical value of miR-19b-3p in sepsis patients, and explored its role in regulating inflammatory responses in HUVECs cells. Sepsis is a condition caused by the inadequate response to the infection, leading to organ dysfunction. Sepsis is a systemic inflammatory reaction syndrome, accompanied by acute inflammatory responses, with the release of multiple inflammatory factors such as TNF-α and IL-6 [2]. Several studies have discussed the occurrence of endothelial dysfunction during sepsis, which can further lead to thrombotic microangiopathy (TMA), remote organ dysfunction and death [3,4,5]. The management of sepsis mainly focuses on containing the infection through source control and antibiotics plus organ function support [6]. The immediate diagnosis and intervention are of great significance for the favorable prognosis of sepsis patients
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