Clinical significance of measurement of changes in EBC leukotriene C4,8-isoprostane and nitrate levels in intermittent and persistent asthma patients

Abstract

Objective To investigate the clinical significance of changes of leukotriene C4 (LTC4),8-isoprostane(8-ISO),nitrate levels in condensate of exhaled breath condensates (EBC) both before and after treatment in patients with asthma.Methods Enzymatic and colorimetric method of 30 cases of patients with asthma LTC4,8-in EBC ISO,nitrate for testing,30 cases are healthy control.Results LTC4 in patients with asthma is higher than the control group (55.17 ng/L than 17.95 ng/L,P ＜0.01),the persistent asthma is higher than Intermittent asthma (67.38 ng/L than 41.21 ng/L,P ＜0.01),the persistent asthma is higher than the control group (67.38 ng/L than 17.95 ng/L,P ＜0.01),the intermittent asthma is higher than the control group (41.21 ng/L than 17.95 ng/L,P ＜0.01),the untreated patients is higher than the treated group (55.17 ng/L than 38.36 ng/L,P ＜0.01).8-ISO in patients with asthma is higher than the control group(13.41 ng/L than 6.93 ng/L,P ＜0.01),the persistent asthma is higher than the control group to high (14.29 ng/L than 6.93 ng/L,P ＜0.01),the intermittent asthma is higher than the control group(12.40 ng/L than 6.93 ng/L,P ＜0.05).Nitrate in patients with asthma is higher than the control group (4.17 ng/L than 3.20 ng/L,P ＜0.01),the persistent asthma is higher than the control group (4.50 ng/L than 3.20 ng/L,P ＜0.01).Conclusions LTC4,8-1SO,nitrate levels can reflects the airway inflammation in patients with asthma and montelukast drugs can control and relieve the airway inflammation. Key words: Asthma ; Exhaled breath condensates ; Leukotriene C4 ; 8-isoprostane; Montelukast