Cysteinyl leukotriene and 8-isoprostane in exhaled breath condensate of asthmatic children

Abstract

Objective To analyze the change of cysteinyl leukotriene (Cys-LTs) levels and 8-Isoprostane (8-iso-PG) levels in the exhaled breath condensate (EBC) of asthmatic children from acute exacerbation to clinical remission, and investigate the role of the detection of Cys-LTs and 8-iso-PG in EBC in its severity and pathogenesis, and explore the relationship between the Cys-LTs and 8-iso-PG through measuring Cys-LTs levels and 8-iso-PG levels in the EBC of asthmatic children. Methods The outpatient or inpatient asthmatic children of the pediatrics and a group of healthy children were studied.All subjects' EBC were collected by the R-Tube produced by American Respiratory Research.The concentration of Cys-LTs and 8-iso-PG in EBC were measured by enzyme-linked immunosorbent assay(ELISA), and compared among children in asthmatic exacerbation, asthmatic remission, and healthy condition.The relevance of their change would be explored at the same time. Results (1)Cys-LTs levels in EBC were higher in asthma exacerbation, compared to healthy controls(P 0.05). (2)8-iso-PG levels was higher in asthmatic exacerbation compared to asthmatic remission (P<0.05). Moreover, the 8-iso-PG levels were significantly higher in asthmatic remission than in healthy controls(P<0.05). (3)Through the relevance analysis of the Cys-LTs and 8-iso-PG levels in EBC among the three groups, Cys-LTs levels in EBC of asthmatic exacerbation significantly were correlated with 8-iso-PG levels (n1=35, r1=0.61, P<0.05), while there was no significant correlation between 8-iso-PG levels and Cys-LTs levels in asthmatic remission. Conclusion The increase of 8-iso-PG levels in EBC of bronchial asthmatic patients correlates with the disease and its control.Therefore, 8-iso-PG can be an objective indicator for asthmatic diagnosis and healing efficacy.Cy-LTs levels increase in the EBC of bronchial asthmatic according to disease severity.The two levels correlate during asthmatic exacerbation, indicating that a link between airway oxidative stress and inflammation among asthmatics. Key words: Children; Bronchial asthma; Exhaled breath condensate; Cysteinyl leukotrien; 8-Isoprostane