Abstract
BackgroundAnkyrin repeat domain 12 (ANKRD12), is encoding a 224 kDa nuclear protein and most conserved at its N-terminal ankyrin repeats region and the C-terminal co-activator interacting domain. The aim of this study was to investigate the ANKRD12 mRNA expression in colorectal cancer (CRC) tumor tissues and the normal adjacent mucosa and its potential relevance to clinicopathological characteristics and prognosis.MethodsSurgical specimens of tumor tissues (n = 68) and adjacent normal mucosa (n = 51) were obtained from CRC patients. The ANKRD12 mRNA expression was measured by quantitative real time reverse transcriptase polymerase chain reaction. The relationship between ANKRD12 mRNA expression and clinicopathological features was analyzed by appropriate statistics. Kaplan–Meier analysis and Cox proportional hazards regression models were used to investigate the correlation between ANKRD12 expression and prognosis of CRC patients.ResultsThe relative mRNA expression of ANKRD12 were significantly lower in CRC tumor tissues than in the normal adjacent mucosa (P < 0.001), and the cases with low ANKRD12 expression showed a higher frequency of liver metastasis (P = 0.015). Kaplan–Meier analysis indicated that patients (CRC without liver metastasis) with low ANKRD12 expression had poor overall survival (P = 0.041). Multivariate analysis showed that low ANKRD12 expression was an independent predictor of overall survival.ConclusionThis study revealed that ANKRD12 mRNA were down regulated in CRC tumor tissues and low ANKRD12 expression was correlated with liver metastasis and poor survival of CRC patients.
Highlights
Ankyrin repeat domain 12 (ANKRD12), is encoding a 224 kDa nuclear protein and most conserved at its N-terminal ankyrin repeats region and the C-terminal co-activator interacting domain
ANKRD12 mRNA expression in colorectal cancer and normal adjacent mucosa By qRT-PCR, we showed that ANKRD12 expression in cancer tissues were significantly lower ( P < 0.001) than those in the normal adjacent mucosa (Figure 1)
ANKRD12 mRNA expression and prognosis of colorectal cancer (CRC) patients Overall survival curves were plotted according to ANKRD12 mRNA expression by the Kaplan–Meier method
Summary
Ankyrin repeat domain 12 (ANKRD12), is encoding a 224 kDa nuclear protein and most conserved at its N-terminal ankyrin repeats region and the C-terminal co-activator interacting domain. Ankyrin repeats-containing cofactor (ANCO) proteins contain both ankyrin repeat and transcriptional repression/activation domains [2-4], including cyclin-dependent kinase inhibitors, transcription factors and cytoskeleton organizers [5]. These proteins were first identified in two yeast cell-cycle regulators, Swi6P and CDC10P, and in the Notch and LIN-12 development regulators [6]. Research showed that ANKRD11 was a p53 coactivator and involved in a regulatory feedback loop with p53 in breast cancer [10]. All of these showed ANKRD11 is a putative tumoursuppressor gene
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