Clinical significance and expression of miR-574-5p in patients with gastric cancer

Abstract

Objective To investigate the expression level and clinical significance of micro RNA-574-5p (miR-574-5p) in gastric carcinoma. Methods From October 2010 to October 2017, 64 specimens of gastric cancer and adjacent tissues from Zhongnan Hospital were collected.Real time fluorescence quantitative technique (RT-PCR) was used to detect the expression of miR-574-5p in specimens. Results The expression of miR-574-5p in gastric cancer tissues was (2.37±0.69)ng/μL, and the expression of miR-574-5p in the para cancerous tissues was (0.36±0.11)ng/μL, the difference was statistically significant (t=25.32, P=0.000). The expression levels of miR-574-5p in patients with the size of tumor size>5cm[(3.04±0.74)ng/μL], TNM staging Ⅲ-Ⅳ[(3.36±0.76)ng/μL], depth of invasion T3-T4[(3.29±0.72)ng/μL], low differentiation[(3.34±0.74)ng/μL], and distant metastasis[(3.39±0.78)ng/μL], were higher than those of tumor size less than 5cm[(2.05±0.68)ng/μL], TNM stage Ⅰ-Ⅱ phase[(2.02±0.61)ng/μL], depth of invasion T1-T2[(2.07±0.64)ng/μL], middle and high differentiation[(2.04±0.65)ng/μL]and no distant metastasis[(2.01±0.68)ng/μL], the differences were statistically significant (t=4.29, 4.68, 4.52, 4.62, 4.79, all P<0.01). Kaplan-meier analysis showed that the 5-year survival rate of patients with high expression of miR-574-5p in gastric cancer was only 16%, which was significantly lower than that in patients with low expression of miR-574-5p (58%), and the difference was statistically significant (χ2=5.872, P=0.001). Conclusion miR-574-5p expression in gastric carcinoma is high.The level of miR-574-5p expression in gastric carcinoma is related to the size of tumor, TNM stage, depth of invasion, degree of differentiation and distant metastasis.The 5-year survival rate of the patients with high expression of miR-574-5p is low. Key words: Stomach neoplasms; Nucleic acids; Pathology, clinical; Neoplasm staging; Neoplasm metastasis; Cell differentiation; Lymphocyte, tumor-infiltrating