Clinical significance and expression of circular RNA circ_0076704 in human gastric carcinoma

Abstract

Objective To investigate the clinical significance of circular RNA circ_0076704 RNA in human gastric carcinoma. Methods Human gastric cancer and matched normal gastric tissue were collected, and circ_0076704 were detected by fluorescence quantitative real-time polymerase chain reaction assay. The relation of between circ_0076704 expression and patient’s sex, age, tumor location, tumor diameter, histological differentiation, depth of tumor invasion, lymph node metastasis, distant metastasis, TNM staging and carcinoembryonic antigen (CEA) levelv and helicobacter pylori (HP) infection and other clinicopathological factors was analyzed, respectively. Results The expression of circ_0076704 was not significantly correlated with patient sex (χ2=1.269, P=0.260), age (χ2=0.139, P=0.709), tumor location (χ2=0.543, P=0.709), tumor diameter (χ2=2.222, P=0.136), degree of tissue differentiation (χ2=4.484, P=0.106), CEA level (χ2=0.135, P=0.714) and HP infection (χ2=0.834, P=0.361), but with the depth of tumor invasion (χ2=11.349, P=0.010). Lymph node metastasis (χ2=11.929, P=0.008), distant metastasis (χ2=10.909, P=0.001), TNM stage (χ2=15.503, P=0.001) and five year survival (χ2=7.519, P=0.006) were significantly correlated. Further studies from ROC curves showed that circ_0076704 sensitivity and specificity were 0.90 and 0.85, respectively. Conclusion Circ_0076704 is highly expressed in gastric cancer tissues and could be used as a molecular marker for gene diagnosis and treatment of gastric cancer. Key words: Gastric carcinoma; Tumor maker; Circular RNA; Significance