Abstract
Kidney plays a central role in maintaining the composition of body fluids by regulating water, NaCl, acid base, and solute reabsorption and excretion, respectively. The study was done to investigate the physiological role of thiamine in regulation of renal response to metabolic acidosis induced by NH 4 Cl in adult male rats. For this experiment, fifty rats were used. They were divided into five groups. Control rats received basal diet; rats fed on basal diet mixed with NH 4 Cl (4g NH 4 Cl/100g diet) to induce severe metabolic acidosis, rats fed on basal supplemented diet with thiamine (600 mg/kg diet), and rats fed on basal supplemented diet with thiamine before and after induction of metabolic acidosis by NH 4 Cl for 14 days. The results showed that the plasma levels of chloride, urea, and creatinine were significantly elevated in metabolic acidosis induced by NH 4 Cl. Thiamine supplementation at high dose before or after induction improved the chloride values. Feeding diets supplemented with thiamine modulated the plasma sodium and bicarbonate values. Supplementation with vitamin B1 as preventive agent significantly restored these changes to near control value and when used as curative agent improved plasma creatinine and urea levels. Urinary pH and potassium levels were decreased significantly in metabolic acidotic rats when compared to all experimental groups. Urinary ammonia and aldosterone levels were decreased by thiamine supplementation as protective agent. Supplementation with vitamin B1 as preventive and curative agents, restored the affected parameters and regulate the response of kidney to metabolic acidosis induced by ammonium chloride.
Highlights
Metabolic acidosis (MA) refers to the condition where the acid base balance of the body is disrupted due to an increased production of acid or the reduced excretion and decreased production of bicarbonates (JONES; WALTER, 2007).Sometimes, metabolic acidosis can occur even without the excessive production of acids, when the kidney fails to excrete them through urine, which can be a symptom of renal failure (NOWIK et al, 2010)
Metabolic acidosis can occur even without the excessive production of acids, when the kidney fails to excrete them through urine, which can be a symptom of renal failure (NOWIK et al, 2010)
The concentration of plasma chloride was significantly (p
Summary
Metabolic acidosis can occur even without the excessive production of acids, when the kidney fails to excrete them through urine, which can be a symptom of renal failure (NOWIK et al, 2010). The kidney plays a central role in maintaining the composition of body fluids by regulating water, NaCl, acid base, and solute reabsorption and excretion, respectively (QUENTIN et al, 2004). Metabolic acidosis induced by NH4Cl has been associated with decreased reabsorption of NaCl and water in the proximal tubule of rat (FAROQUI et al, 2006). Thiamine is especially important in glucose metabolism. It acts as a cofactor for enzymes that break down glucose for energy production (Figure 10.3). The brain and heart are most affected by a deficiency in thiamine (SHRADHA et al, 2007)
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