Abstract
BackgroundAberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI.MethodsDetection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b.ResultsmiR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %.ConclusionsmiR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction.
Highlights
Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI)
The result indicated that miRNA-208b was significantly increased in AMI cases compared with normal healthy people, while miR-26a and miR-191 were decreased
Variation in expression of miR-26a, miR-191, and miR-208b with time We showed that the relative expression of miR-26a, miR-191, and miR-208b changed over time
Summary
Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). If a sensitive and specific biomarker could be found to differentiate between AMI and some other causes of chest pain, it miRNAs are a class of endogenous, single-stranded, 19–22-nucleotide, non-coding RNAs. From the latest database, there are >2000 mature miRNAs associated with the expression of human protein mRNA [8]. Some researchers have reported that aberrant expression of miRNAs in tissues or cells could promote various diseases, such as cancer and cardiovascular diseases [12,13,14,15]. Some studies have indicated that circulating miRNAs in the plasma and serum could act as biomarkers for AMI diagnosis and treatment, such as miR-21, miR-301, miR-328, and miR-34 [16,17,18]
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