Abstract
High resolution melting (HRM) analysis is a real-time PCR based technique which can detect DNA sequence polymorphism. Immediately after routine PCR with saturated fluorescent dye, melting transition of the amplicon was monitored and fluorescence was collected. According to the different melting curve, single base differences can be distinguished. HRM is a low-cost, single-step, closed-tube, accurate and rapid method, which has aroused general concern. Rapid and sensitive detection of the clinical pathogens is critical to diagnosis of infectious diseases. However, clinical research on application of HRM in infectious disease pathogen identification and drug resistance, especially melting curve analysis based bedside technology integration will provide strong support for the diagnosis and treatment of infectious diseases. (Chin J Lab Med, 2017, 40: 84-87) Key words: Bacterial infections; Drug resistance, bacterial; Microbial sensitivity tests; High resolution melting
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