Classical taxonomy studies of medicinally important Ipomoea leari

Abstract

Background:Ipomoea leari which belongs to the family Convolvulaceae is an unexplored medicinal plant in the Indian medicinal system. According to ethnobotanical information, the whole plant is used for various disorders such as anti-inflammatory, psychotomimetic and anticancer activities. The current study seeks to standardize the parameters for this herb.Materials and Methods:The identification of the pharmacognostical, morphoanatomical characters of Ipomoea leari (leaf, stem and root) were carried out in terms of organoleptic, macroscopic, microscopic, physicochemical, florescence and phytochemical analyses. Physicochemical parameters such as total ash, moisture content and extractive values were determined by World Health Organization (WHO) guidelines. The plant material was fixed in formalin-5 mL + acetic acid-5 mL + 70% ethyl alcohol-9 mL (FAA) and dehydrated with graded series of tertiary-butyl alcohol. Toluidine blue, a polychromatic stain was used for staining the sections and then whole components were observed with Nikon lab photo device with microscopic units.Results:Microscopically, leaf consists of prominent midrib and the lamina, both having dorsiventral symmetry. The stomata are actinocytic. The stem consists of an epidermal layer of one cell thickness, wide cortex, vascular cylinder and wide pith. The root measuring 1.6 mm thick was studied. It consists of uniformly thick and continuous periderm, wide cortex and thick vascular cylinder. Qualitative analysis revealed the presence of carbohydrates, flavonoids, glycosides, steroids and phenols. The pharmacognostic studies were carried out in terms of macroscopic, phytoconstituent and chromatographic analyses of Ipomoea leari. Various standard methods were adopted to carry out the investigation.Conclusion:The results of the present study provide valuable pharmacognostic information of Ipomoea leari for its identification. Our result's suggest that Ipomoea leari is a promising candidate as an adjuvant therapy in various disorders and preparation of monograph.