Abstract
It is well known that chemotactic agents active Na+/H+ exchanger, increasing intracellular pH of neutrophils, but their effect on bicarbonate transporters have not been established yet. To study the effect of fMLP on the activity of Cl−/HCO3- exchange, the rate of pH recovery after acute Cl− readmission in cell subjected to an alkaline load by CO2 washout in a Cl-free medium was measured. The activity of the exchanger was reduced to 72% of control when cells were pre-incubated for 5min with 0.1μM fMLP and reached 48% of control in steady state after acute exposure. After extracellular bicarbonate or TMA addition the rate recovery of intracellular pH was reduce at 72% and at 84%, respectively. The inhibitory effect on the intracellular pH recovery was not affected by blockers of Na+/H+ exchange. We conclude from these studies that an increase of pHi produced for this chemotactic agent is facilitated by the simultaneous activation of Na+/H+ exchange and inhibition of Cl−/HCO3- exchange in neutrophils.
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