Citric acid production by selected mutants of Aspergillus niger from cane molasses

Abstract

The present investigation deals with citric acid production by some selected mutant strains of Aspergillus niger from cane molasses in 250 ml Erlenmeyer flasks. For this purpose, a conidial suspension of A. niger GCB-75, which produced 31.1 g/l citric acid from 15% (w/v) molasses sugar, was subjected to UV-induced mutagenesis. Among the 3 variants, GCM-45 was found to be a better producer of citric acid (50.0 ± 2a) and it was further improved by chemical mutagenesis using N-methyl, N-nitro- N-nitroso-guanidine (MNNG). Out of 3,2-deoxy- d-glucose resistant variants, GCMC-7 was selected as the best mutant, which produced 96.1 ± 1.5 g/l citric acid 168 h after fermentation of potassium ferrocyanide and H 2SO 4 pre-treated blackstrap molasses in Vogel’s medium. On the basis of kinetic parameters such as volumetric substrate uptake rate ( Q s), and specific substrate uptake rate ( q s), the volumetric productivity, theoretical yield and specific product formation rate, it was observed that the mutants were faster growing organisms and produced more citric acid. The mutant GCMC-7 has greater commercial potential than the parental strain with regard to citrate synthase activity. The addition of 2.0 × 10 −5 M MgSO 4 · 5H 2O into the fermentation medium reduced the Fe 2+ ion concentration by counter-acting its deleterious effect on mycelial growth. The magnesium ions also induced a loose-pelleted form of growth (0.6 mm, diameter), reduced the biomass concentration (12.5 g/l) and increased the volumetric productivity of citric acid monohydrate (113.6 ± 5 g/l).