Abstract
The combination citrate-saponin-acid procedure with its internal modifications of each of the separate standard methods, viz. , citrate, saponin, and Knott's, was developed to produce greater yields of microfilariae from equivalent amounts of peripheral blood of infected patients, especially those with scanty numbers of organisms, than the total yields recoverable by the three standard methods separately. Packed erythrocytes from 10.0 ml. of citrate-treated whole blood were destroyed through the action of a 0.5% saponin solution, after which centrifugation facilitated the recovery under low power magnification of motile microfilariae in wet mounts made from the sediment. Preparations designated mini-Knott's were made as wet mounts from two drops of sediment and were first examined for motile microfilariae and subsequently mixed in place with several drops of 1.0% acetic acid solution before drying and staining. Examination of these slides permitted the demonstration of concentrated numbers of straightened microfilariae under low power and identification under oil immersion magnification. Eighteen cases are reported.
Published Version
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