Cisplatin loaded Nano Lipid Carriers for the Treatment of Skin Cancer

Abstract

The aim of the present study was to develop a Cisplatin loaded NLCS to enhanced and effective delivery of drug to the skin cancer. NLCS bearing Cisplatin were prepared by emulsification solvent diffusion method. The formulated NLCS were characterised for average particle size, polydispersity index (PDI) Zeta potential, entrapment efficiency and in vitro drug release study. The prepared formulations were studied for its in vitro cell line and cell uptake study. It was revealed that the average size of NLCS was found 172.2±3.2, PDI was 0.135, % entrapment efficiency was found 68.9±2.04 and Zeta potential was found-15.3±1.3. In-vitro release determined by Frenz diffusion cell was found 63.7±2.8% after 72 hr. MTT assay shows that Cisplatin loaded NLCS were giving more cytotoxity as compare to plain drug. The cell uptake study was found enhanced uptake of FITC loaded NLCS in comparison to plain FITC. Cisplatin and Cisplatin loaded NLCS showed 22.3±1.2 and 32.8±0.9 growth inhibition respectively after 48h upon incubation at 0.5 μg/mL concentration (p<0.05). The result of the studies was concluded that NLCS can be use as impending drug delivery system which may enhance the drug uptake and maintain the drug level for longer period of time and it is potential carrier system which can be use for the treatment of skin diseases like cancer.