Circulating miR-21-5p, miR-146a-5p, miR-320a-3p in patients with atrial fibrillation in combination with hypertension and coronary artery disease

S V Vasiliev,D A Stonogina,E I Sharova,A V Zhelankin,A S Akselrod,E V Generozov,D Yu Schekochikhin

doi:10.15829/1728-8800-2022-2814

Abstract

Aim. To study the plasma profiles of circulating extracellular microribonucleic acids (miRNAs), potentially including in pathogenesis of cardiovascular diseases, in patients with atrial fibrillation (AF) in combination with hypertension (HTN) or coronary artery disease (CAD).Material and methods. The study included patients with AF in combi nation with HTN (n=21) or CAD (n=10), as well as following control groups: patients with uncomplicated HTN without AF (n=28), patients with stable CAD without AF (n=10) and healthy individuals (n=30). MiRNA samples were isolated from blood plasma of the study participants. MiRNAs were detected by TaqMan quantitative polymerase chain reaction assay. The relative plasma levels of five candidate miRNAs were estimated relative to the reference miR-16-5p.Results. Among the analyzed circulating plasma miRNAs, a higher level of miR-320a-3p was associated with AF, while increased levels of miR 146a-5p and miR-21-5p are potentially associated with presence of both AF and CAD.Conclusion. Differences were found in the plasma miRNA profiles (miR-21-5p, miR-320a-3p, miR-146a-5p) between patients with AF, regardless of concomitant disease (CAD or HTN), and healthy individuals in the control group.

Highlights

Большее напряжение Адаптационный потенциал (АП), характеризующего состояние адаптации сердечно-сосудистой системы, отмечено среди жительниц Азиатского Севера
A significant disadaptation was revealed in residents of Asian North compared to European North as follows: the proportion of persons with poor adaptation increases from 16% in European North to 26% in Asian North in fertile women, while the proportion of persons with adaptation failure increases from 9 to 23% in postmenopausal women
The strongest relationships between hormonal parameters and Adaptive potential (AP) level were found in postmenopausal women: in European North, adaptation impairment is associated with higher levels of testosterone at lower concentrations of follicle-stimulating hormone, luteinizing hormones (LH) and sex hormone-binding globulin; in Asian North, adaptation failure is associated with lower values of LH, prolactin, testosterone, and dopamine

Summary

Introduction

А. Адаптационный потенциал системы кровообращения и его взаимосвязь с половыми гормонами и уровнем дофамина у женщин Архангельской области и Ямало-Ненецкого автономного округа. A. Adaptive potential of cardiovascular system and its relationship with sex hormones and dopamine levels in women of Arkhangelsk Oblast and Yamalo-Nenets Autonomous Okrug. Проживающих на ЕС, 3% (2/58) имеют хорошую адаптацию системы кровообращения, 81% (47/58) испытывают функциональное напряжение механизмов адаптации и 16% (9/58) имеют неудовлетворительную адаптацию.

Results

Conclusion