Circular RNA circ_103820 suppresses lung cancer tumorigenesis by sponging miR-200b-3p to release LATS2 and SOCS6

Yongbin Chi,Wenlong Zheng,Xiaoxue He,Guangyu Bao,Lifeng Wu,Yan Shen,Mingming Jin,Xudong Yin,Ruyi Gan

doi:10.1038/s41419-021-03472-7

Abstract

A growing number of circular RNAs (circRNAs) have been identified and verified in several cancers. However, highly efficient therapeutic methods based on circRNAs in lung cancer remain largely unexplored. In the present study, we identified a novel circular RNA, hsa_circ_103820, based on Gene Expression Omnibus (GEO) data. Functionally, overexpression of hsa_circ_103820 showed significant inhibitory effects on the proliferation, migration and invasion of lung cancer cells, and knockdown of hsa_circ_103820 played promoting roles. Regarding the mechanism, we revealed that miR-200b-3p was a direct target of hsa_circ_103820 and that LATS2 and SOCS6 were the downstream target genes of miR-200b-3p. Therefore, we identified a novel potential tumor suppressive function of hsa_circ_103820 in lung cancer.

Highlights

Lung cancer is estimated to account for approximately one-fifth of all deaths due to malignant tumors worldwide[1]
We identified a novel circular RNA, hsa_circ_103820, which is located at chr5:38523520–38530768 and is encoded by leukemia inhibitory factor receptor (LIFR) gene exons that was significantly underexpressed in lung cancer compared to normal controls
To identify the potential circRNAs that might participate in the tumorigenesis of lung cancer, we searched “circRNA” and “lung cancer” in the Gene Expression Omnibus (GEO) dataset and discovered the GSE101586, GSE101684, and GSE112214 datasets

Summary

Introduction

Lung cancer is estimated to account for approximately one-fifth of all deaths due to malignant tumors worldwide[1]. Due to the special stable structure, circRNAs are not degraded by RNA enzymes, which are evolutionarily conserved[7]. Based on these characteristics, circRNAs, as biomarkers, might provide new ideas for drug development and new directions for disease research[8]. We aimed to select the expression profiles of differentially expressed circRNAs between lung cancer and adjacent normal lung tissues by adopting the data in the Gene Expression Omnibus (GEO) datasets. We identified a novel circular RNA, hsa_circ_103820 (hsa_circ_0072309), which is located at chr5:38523520–38530768 (human GRCh37/hg19) and is encoded by leukemia inhibitory factor receptor (LIFR) gene exons that was significantly underexpressed in lung cancer compared to normal controls. The Official journal of the Cell Death Differentiation Association

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