Abstract
The miniaturization of flow focuser is a challenge in developing microflow cytometers. Most previously reported microfluidic cell focusers require complex structures or external force fields to achieve the 3D cell focusing. Herein, we propose a novel circular-channel particle focuser utilizing viscoelastic focusing. The circular-channel particle focuser is fabricated using a simple and low-cost microwire molding technique. Whole PDMS channels with perfect circular cross-sections can be fabricated using this protocol. We then characterize the particle focusing performances in our circular-channel particle focuser and discuss the effects of particle size, operating flow rate, cross-sectional dimension and fluid rheological property on particle focusing. The experimental results show that a perfect single-line focusing can be achieved exactly at the channel centerline. Finally, our circular-channel particle focuser is employed for the focusing of blood cells. As it offers special advantages of simple structure, easy fabrication, and sheathless operation, our circular-channel particle focuser may serve as a potential flow focuser for microflow cytometers.
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