Cinnamon Bark Volatile Oils Separation and Determination Using Solid-Phase Extraction and Gas Chromatography

Abstract

One of the SPE method is reversed phase separation which involve a polar usually aqueous or moderately polar sample matrix (mobile phase) and a nonpolar stationary phase. The analyte of interest is typically mid-to non polar. Several SPE materials are in the reversed phase category including C18. The hydrophilic silanol groups at the surface of the raw silica packing have been chemically modified with hydrophobic alkyl functional groups by reaction with the corresponding silanes. Retention of organic analytes from polar solutions on to the SPE materials is due primarily to attractive forces between the carbon-hydrogen bonds in the analyte and the functional group in the silica surface. These nonpolar-nonpolar attractive forces are van der waals forces. In this work, at first the aqueous solutions of Cinnamomum zeylanicum Blume bark were obtained by hydrodistillation and superheated water extraction (SWE) methods, then main the volatile compounds were extracted from aqueous solutions by solid phase extraction (SPE) method. This method involves adsorption of the desired components on C18 SEP PAK (Monofunctional -Si (CH3)2C18H37), followed by desorption in the desorption chamber, using minimum volume solvent as the mobile phase. Solid phase extraction conditions optimized on C18 SEP PAK classic cartridge. Different solvent including acetone, hexane, ethyl acetate, ethanol, and mix methanol: water were examined. A good separation of Ecinnamaldehyde was obtained on the adsorbent column. The amount of extracted essential oil composition of C. zeylanicum by SPE, evaluated in different conditions. Gas chromatography/mass spectrometry and gas chromatography were used to identify and quantity the volatile compound composition. GC analysis showed that the maximum yield of E-cinnamaldehyde obtained by methanol: water in (70:30 v/v) ratio by SPE extraction. At the optimum condition, the GC results indicated that E-cinnamaldehyde was the highest area percentage of 79.60% in the volatile oils extracted by hydrodistillation and 88.50% by superheated water extraction.