Cinnamaldehyde and α-terpineol inhibit the growth of planktonic cultures of Candida albicans and non albicans

Loyse Martorano Fernandes,Leopoldina De Fátima Dantas De Almeida,Yuri Wanderley Cavalcanti,Mariana Cavalcanti Lacerda

doi:10.33448/rsd-v10i10.19027

Loyse Martorano Fernandes, Leopoldina De Fátima Dantas De Almeida + Show 2 more

Open Access

https://doi.org/10.33448/rsd-v10i10.19027

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Abstract

Agents based in natural products have been investigated for the treatment of oral candidiasis. This study aims to evaluate the antifungal effect of phytoconstituent cinnamaldehyde and α-terpineol in planktonic cultures of Candida albicans, Candida glabrata, Candida krusei and clinical isolates of C. albicans. Reference strains of C. albicans (ATCC 90028 and ATCC 60193), C. glabrata (ATCC 2001), C. krusei (ATCC 34135) and four clinical isolates were used. Nistatin 100,000UI was used as a positive control. After preparation of the inoculum (1 × 103 CFU / mL), serial microdilution technique was performed using RPMI 1640 medium. Results: in reference strains, the MIC for α-terpineol ranged from 312,5 μg / mL (C. albicans 90028) to 40 μg / mL (C. krusei); and the cinnamaldehyde ranged from 40 μg / mL (C. albicans 90028, C. albicans 60193 and C. glabrata) to 20 μg / mL (C. krusei). Whereas for clinical strains, the MIC for α-terpineol ranged from 156 μg / mL to 78 μg / mL and cinnamaldehyde ranged from 78 μg / mL to 40 μg / mL. Therefore, the cinnamaldehyde and α-terpineol present an inhibitory effect against planktonic cultures of Candida albicans and not albicans.

Highlights

Oral candidiasis is an opportunistic fungal infection caused by poor oral hygiene conditions, and the presence of biofilm (Hellstein et al, 2019)
Candida albicans is the prevalent fungi in this infection (Hellstein et al, 2019), other Candida spp are related with this disease, as Candida glabrata and Candida krusei (Hu et al, 2019)
Concerning the reference strains, the minimum inhibitory concentration (MIC) of cinnamaldehyde was found to range from 40 μg / ml to 20 μg / ml depending on the strain evaluated

Summary

Introduction

Oral candidiasis is an opportunistic fungal infection caused by poor oral hygiene conditions, and the presence of biofilm (Hellstein et al, 2019) This disease is prevalent in patients with immunosuppression (Suryana et al, 2020), diabetic mellitus (Tretin et al, 2017) and denture wearers (Radovic et al, 2014). Candida albicans is the prevalent fungi in this infection (Hellstein et al, 2019), other Candida spp are related with this disease, as Candida glabrata and Candida krusei (Hu et al, 2019) These species form communities of microorganisms embedded within an extracellular matrix (Silva et al, 2012), which facilitates the epithelial invasion, protects microbial cells from host immune responses, promotes protection to biofilm by limiting the penetration of substances through the matrix. Patients with living with HIV or with immunological deficits, must be treated as fast as possible, because this infection could develop a nosocomial infection, which may lead to death (Kabwe et al, 2016)

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