Abstract
Background: Cimetidine reverses immunosuppression following trauma, however, its effect on pure haemorrhagic shock is unknown. Methods: Mice sensitized by injection of sheep red blood cells (SRBCs), were subjected to cardiac puncture and randomized to a control group-A ( n=11) and three shock groups (35% of blood volume extracted): group-S had no treatment ( n=16), group-CP received cimetidine 50 mg/kg intraperitoneally ( n=16), group-CW received oral cimetidine (200 mg/kg per day, n=16). After 5 days, animals were challenged by injection of SRBCs into the foot-pad of the right hind paw (same volume of saline was injected into left paw). Foot-pad thickness ratios (FPTRs) were determined at 16 and 40 h, and inflammatory response was assessed histologically. Results: At 16 h, FPTRs were greater in group-CW than group-S ( P=0.01). There were no differences at 40 h. More animals in groups-CP and -CW had grade 3/4 inflammation, whilst group-S had the least inflammatory response (NS). Conclusions: Cimetidine prevents suppression of delayed hypersensitivity in this model.
Published Version
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