Cibacron blue immobilized poly(glycidyl-methacrylate) nanobeads for albumin removal in proteome studies

Abstract

A new approach to albumin removal for proteome studies involving human plasma samples was presented with dye affinity poly glycidyl methacrylate (PGMA) nanobeads in an average size of 45 nm. The specific surface area of PGMA nanobeads was calculated as 2616 m2/g. Cibacron Blue F3GA (CB) was immobilized onto PGMA nanobeads as dye ligand, and CB immobilized PGMA (CB-PGMA) nanobeads were characterized by a serial processing. Albumin depletion efficiency of CB-PGMA nanobeads was investigated in human plasma sample and confirmed by two-dimensional gel electrophoresis.