Abstract
Recent studies correlate chronic Toxoplasma gondii (T. gondii) infection with behavioral changes in rodents; additionally, seropositivity in humans is reported to be associated with behavioral and neuropsychiatric diseases. In this study we investigated whether the described behavioral changes in a murine model of chronic toxoplasmosis are associated with changes in synaptic plasticity and brain neuronal circuitry. In mice chronically infected with T. gondii, magnetic resonance imaging (MRI) data analysis displayed the presence of heterogeneous lesions scattered throughout all brain areas. However, a higher density of lesions was observed within specific regions such as the somatosensory cortex (SSC). Further histopathological examination of these brain areas indicated the presence of activated resident glia and recruited immune cells accompanied by limited alterations of neuronal viability. In vivo diffusion-tensor MRI analysis of neuronal fiber density within the infected regions revealed connectivity abnormalities in the SSC. Altered fiber density was confirmed by morphological analysis of individual, pyramidal and granule neurons, showing a reduction in dendritic arbor and spine density within the SSC, as well as in the hippocampus. Evaluation of synapse efficacy revealed diminished levels of two key synaptic proteins, PSD95 and synaptophysin, within the same brain areas, indicating deficits in functionality of the synaptic neurotransmission in infected mice. Our results demonstrate that persistent T. gondii infection in a murine model results in synaptic deficits within brain structures leading to disturbances in the morphology of noninfected neurons and modified brain connectivity, suggesting a potential explanation for the behavioral and neuropsychiatric alterations.
Highlights
Toxoplasma gondii (T. gondii) is a widespread intracellular parasite that is able to infect all warm-blooded species
Implications and future directions These findings indicate that, upon latent infection with T. gondii, marked neuroanatomical changes occur in central nervous system (CNS) regions that are relevant for normal behavior, and establish a murine model for translational studies of chronic toxoplasmosis
The neuroanatomical changes reported here are similar to those previously reported in individuals with neuropsychiatric diseases such as schizophrenia-like disorders, which have been associated with T. gondii infection in some studies
Summary
Toxoplasma gondii (T. gondii) is a widespread intracellular parasite that is able to infect all warm-blooded species. Human infections occur mainly through oral ingestion of contaminated food or water. 30% of the entire human population is estimated to be infected with T. gondii without major clinical manifestations in immunocompetent individuals (Montoya and Liesenfeld, 2004; Munoz et al, 2011). After dissemination throughout the body, the parasites reach immune-privileged areas such as the central nervous system (CNS) (Ferguson et al, 1991; Dubey, 2009). Latent toxoplasmosis is characterized by bradyzoitecontaining cyst development within neurons, where the parasites stay hidden from the immune system (Lyons et al, 2002; Dubey, 2009)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
