Chronic Intermittent Hypoxia Training on Rat Myocardial Mitochondria Atpase Impact Study

Abstract

To study the chronic intermittent hypoxia training on rat myocardial line grain of ATP content and Na+, K+ ATPase and the influence of Ca2+, magnesium 2+ ATPase. Methods: 32 male Wistar rats as the research object, according to the low oxygen training program can be divided into intermittent group, acute group and the control group, among which chronic intermittent hypoxia training, intermittent simulated altitude 3 km, 2w, then simulated altitude training 5 km, 2w training, daily training 4 h, finally simulated altitude 8 km, placed 4 h. Acute group immediately under simulated conditions of 8 km altitude 4 h; The control group are not hypoxic training. After the expiry of the low oxygen, beheaded executed after, separation of myocardial mitochondria, determination of ATP enzyme activity. Results: (1) atpase chronic group content (9.04 ± 4.71 mg/100 mg of BW, (4.96 − 1.17) in the acute group mg/100 mg of BW and the control group (4.38 ± 0.95 mg/100 mg of BW were significantly higher, significant difference, statistically significant (P < 0.05). (2) chronic group of Na+, K+ ATPase activity (2.55 ± 1.41) mu mol, pro - 1 mg/h, (2.66 ± 1.07) in the acute group mu mol, pro - 1 mg/h and the control group (3.08 ± 1.37) mu mol, pro - 1 mg/h had no significant difference. (3) chronic group of Ca2+, magnesium 2+ ATPase activity (1.17 ± 0.34) mu mol, pro - 1 mg/h, the control group (1.28 ± 0.42) mu mol, pro - 1 mg/h no significant difference, but the acute group (0.58 ± 0.14) mu mol, mg/h significantly higher pro - 1, the difference has statistical significance (P < 0.05). Conclusion: chronic intermittent hypoxia training to ensure the Ca2+, magnesium 2+ ATPase activity has a positive meaning, at the same time can significantly increase the content of ATPase, help to improve myocardial motion function, adapt the rat hypoxic environment.