Abstract
Oncogene activation induced by chromosomal changes is now regarded as one of the most important phenomena during carcinogenesis. We have reproted c- abl activation in a rat leukemia cell line K3D, caused by a secondary chromosomal translocation. Another erythroblastic leukemia cell line D5A1, originally derived from a leukemia induced by 7,12-dimethylbenz(a)anthracene (DMBA) in a Long-Evans rat, is characterized by a marker chromosome 1q+, which also probably occurred as a secondary change. In this cell line, the transcription level of Ha- ras related mRNA increased compared with other cell lines. By the in situ hybridization technique, the c-Ha- ras locus was assigned to 1q43 and the breakpoint 1q+. Because the breakpoint was so near the c-Ha- ras locus on the chromosome, the present system may provide a model of activation of the c-Ha- ras gene brought about by chromosomal translocation.
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