Abstract

The karyotype of 28 families from Shandong, Zhejiang, Guizhou and other 12 provinces exist in half-sib families of ancient Ginkgo biloba gemplasm repository was studied by squashing method with young leaves from these families. Results: The results showed that the number of chromosomes in 28 families was 2n=2x=24. The medium-short chromosomes and the medium-long chromosomes were dominant. The karyotype consists of three types: median region chromosome (m), submedian region chromosome (sm) and subterminal region chromosome (st). 71# family is the most primitive, while the 40# family is the most evolved. Families from southwestern China have a low degree of evolution, while those from eastern and southern China have a high degree of evolution.

Highlights

  • Ginkgo biloba L. is a relict species of Quaternary glaciers, a historical heritage and living fossil, with important economic, ecological and social benefits [1]

  • Collection, preservation and evaluation of ancient Ginkgo biloba germplasm resources are of great significance for the preservation of precious genes of Ginkgo biloba

  • The results showed that the chromosome numbers of 28 half-sib families of ancient Ginkgo biloba were 2n=2x=24 (Figure 1)

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Summary

Introduction

Ginkgo biloba L. is a relict species of Quaternary glaciers, a historical heritage and living fossil, with important economic, ecological and social benefits [1]. Ginkgo biloba has a long history of cultivation in China. It flourished in the south of the Yangtze River in the late Han Dynasty, in the Central Plains in the Tang Dynasty and was widely planted in the Yellow River Valley in the Song Dynasty. As a unique historical and natural landscape, ancient Ginkgo biloba is a precious germplasm resource repository and research material for evolution. Collection, preservation and evaluation of ancient Ginkgo biloba germplasm resources are of great significance for the preservation of precious genes of Ginkgo biloba. Most studies on ancient Ginkgo biloba focused on the investigation of germplasm resources and the rejuvenation of weakened ancient trees [6,7,8], while the exsitu-preservation of ancient Ginkgo biloba system is still lacking

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