Chromosome instability and sister chromatid exchange (SCE) studies in patients with carcinoma of cervix uteri

Abstract

Cytogenetic studies have been carried out using the G-banding technique in peripheral blood lymphocytes of 14 patients with carcinoma of the cervix uteri. Simultaneously, sister chromatid exchange (SCE) was also analyzed in the peripheral blood lymphocytes of these patients, along with those of 20 age-matched control subjects. The frequency of aberrant metaphases is significantly higher in patients with carcinoma of the cervix uteri (7.85%) than in the age-matched controls (3.35%). A large number of chromosome aberrations in lymphocytes of these patients have also been detected. Sister chromatid exchange (SCE) was also analyzed in lymphocytes of 14 patients with carcinoma of the cervix uteri and 20 age-matched control subjects. The mean SCE frequencies were 9.44 ± 0.34 (n = 637) and 6.09 ± 0.24 (n = 900) per metaphase in patients and controls, respectively. The increase of SCE frequency in cancer patients was statistically significant (p < 0.001), but not seen in controls. Our results suggest that patients with carcinoma of the cervix uteri show a degree of chromosomal instability that might be related to a predisposition to neoplasia.