Abstract
SUMMARYThe present communication deals with the study of chromosome behaviour in plants, regenerated in vitro through direct adventitious bud formation from leaf segments in Solanum sarrachoides Sendt. Highest number of adventitious shoots was obtained in MS semisolid medium supplemented with 2iP (2 mgl−1) as the only growth regulator. Addition of IAA was necessary in the later phase of culture to obtain better growth and development of shoots with well-defined leaves, roots, flowers and fruits. Analysis of the pollen mother and root tip cells of the regenerants showed n = 12 and 2n = 24 chromosomes respectively. Hyperdiploids along with the normal diploid cells were also recorded in low percentage.
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