Abstract
A monosomic set of male‐sterile ‘Chris’ (Triticum aestivum L.) with T. timopheevi Zhuk. cytoplasm was used to determine the chromosomal location of Rf‐genes in six male fertility restoring lines (FR‐lines) of T. aestivum L. The six FR‐lines were: R1‐Lee (Nebraska 542437/T. aestivum ‘Lee’), R2‐Sonora 64 (T. timopheevi/2*T. aestivum ‘Marquis’//‘Sonora 64’), R3 (T. timopheevi/3*T. aestivum ‘Marquis’), R4 (amphidiploid T. timopheevi‐Ae. squarrosa/3*T. aestivum ‘Dirk’), R5 (T. Zhukovsky Men. and Er.,/3*T. aestivum ‘Justin’), and T. aestivum ‘Primepi’. The relative proportions of male‐sterile and male‐fertile plants in the F2 and F3 from monosomic F1 indicated that chromosomes 1A and 7D of R1‐Lee, R2‐Sonora 64, R3, R4, and R5 had Rf‐genes. R2‐Sonora 64 and R5 each had a third Rf‐gene on chromosomes 6B and 7B, respectively. Primepi had Rf‐genes on chromosomes 1B and 5D. The inheritance of fertility restoration in control crosses and in some of the noncritical crosses indicated that R1‐Lee, R2‐Sonora 64, and R5 each had three Rf‐genes, and R3, R4, and Primepi each had two. The deficiency of certain chromosomes influenced the penetrance and expressivity of the Rf‐genes in certain crosses.
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