Abstract
Abstract The nucleolar organizer regions (NORs) have often been analyzed in Parodontidae fish using silver staining. In the genus Parodon, the Ag-NORs are used as species chromosomal markers, while in the genus Apareiodon they show a more conserved location. In the present study, the NORs are investigated in three Parodon species (P. hilarii, Parodon sp. and P. tortuosus) using silver and fluorochrome staining and fluorescence in situ hybridization (FISH) with a PCRobtained 18S probe. In addition, the chromosomal 5S rDNA sites are also analyzed with a cloned 5S rDNA probe. It was seen a close correspondence between Ag-NORs, chromomycin positive regions and the 18S rDNA sites in the chromosomes of the three species. A major and a minor 5S rDNA cluster were also abserved in these species. While the 18S and major 5S rRNA genes are located on different chromosome pairs in Parodon sp. and P. hilarii, they are linked in P. tortuosus, although in opposite regions of the chromosome. Both 18S and 5S rDNA sites app...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
