Abstract
Four permanent cell lines derived from malignant human gliomas were karyotyped using Giemsa-trypsin banding. D-65 MG had a stemline with 44 chromosomes, including 11 markers: 1p+, 2q−, 3p−, 3q+, 4p−, 9q−, 11q+, 15q−, 17p+, 21p+, 22q−. The net effect after accounting for fragments in markers was: +8, −10, −16 −X. D-32 MG had chromosome counts 90–91 without a distinct stem karyotype. Modal cells contained from 3 to 5 copies of the normal autosomes and 5 markers: 1q−, 3q−, 7q−, 13q−, 18q−. D-32 MGCl 2 had a complex karyotype containing 78–82 chromosomes. There was no stemline, and modal cells varied from one another primarily in their set of marker chromosomes. A total of 23 markers were seen in this line, 17 of which were present in most modal cells. They were partially characterized as: 1q+, 1q+, 2q−, 5q+, 7p−, 7q−, 8p+, 8p+, 9p+, 12p+, 14q−, 16q+, 19q+, 19p+, a small submetacentric chromosome of undetermined origin and two small isochromosomes, i(Dp or Gp) and i(17p or 18p). A-172 MG had a modal peak of 77 chromosomes within which no two cells were exactly alike. Ten markers seen in modal cells were: 1p−, 4p+, 6p+, 6p+, 6q−, 7p−, 9p−q+, 13q+ 14p+, 22q+. There were no normal copies of chromosomes #1, #6, #9, #14. These four glioma-derived cell lines possess unique karyotypes, but each displays some combination of the numerical and structural deviations generally associated with established glioma lines.
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