Chromium stress influences several parameters of leaf dynamics and morphology in Oryza sativa L. genotypes

Abstract

Chromium (Cr) stress is a prevalent abiotic stress that alters various physiological, growth and metabolic parameters in plants. The toxicity of chromium at concentrations up to 100 µM were studied in two common Indian Oryza sativa genotypes, Lalat upland and Swarnaprabha. More chromium accumulated in roots than in leaves in both genotypes, with Lalat upland taking up more chromium than Swarnaprabha. Higher chromium concentrations reduced various physiological traits including stomatal conductance, transpiration rate, photosynthetic water use efficiency, photosynthetic pigments content in both the genotypes, and this decline was more pronounced in Lalat upland. Increase in vapour pressure deficit, sub stomatal carbon dioxide concentration and malondialdehyde content was detected at 100 µM chromium treated genotypes, however the effects were higher in Lalat upland. Confocal microscopy showed stomatal guard cell deformation, greater stomatal area and closed stomatal aperture in the leaves of 100 µM chromium treated Lalat upland plants. Abscisic acid and Salicylic acid content and expression of NCED1 and NCED2 genes involved in stomata aperture regulation were enhanced in Lalat upland and down regulated in Swarnaprabha under chromium stress. Variation in glandular and non-glandular trichome density and length was found in the leaves of chromium stressed plants in both the genotypes. The results suggest that Lalat upland is more sensitive to chromium toxicity than Swarnaprabha at higher chromium concentrations.