Abstract
This experiment is conducted in vitro to investigate trivalent chromium picolinate affects the proliferation and differentiation of myoblasts. A myoblasts cell line (C2C12) from rats was used in the experiment. These were randomly divided into the control group, the Pic group (50ppb picolinate) and the CrPic group (50ppb chromium picolinate). The differentiation of myoblasts reveals that the number of differentiated myotubes, creatine kinase (CK) activity and the aldolase (ALB) activity do not differ among the three groups (P > 0.05). The activity of hexokinase in the CrPic and Pic groups clearly exceeds that in the control group (P 0.05). Myoblast proliferation was the same across the three groups (P > 0.05), and the quantity of DNA in the control group exceeded that in the Pic group (P < 0.05). The experiment indicated that 200ppb chromium picolinate did not influence the proliferation and differentiation of myoblasts.
Highlights
MATERIALS AND METHODSTrivalent chromium has been recognized as a cofactor of insulin[1,2], and has played a prominent physiological role in humans[3,4] and domestic animals[58]
When the myoblasts reached confluence, the cells were divided into three groups, control, 200ppb picolinic acid (Pic) and 200ppb Cr as chromium picolinate (CrPic)
Trial 2: Proliferation trial: The proliferation trial involved seeding 5×104 cells in each fask, a total of 18 flasks were used, and the cells were divided into control, 200ppb ( g/L) picolinic acid (Pic) and 200ppb Cr as chromium picolinate (CrPic) groups, all of which were incubated for five days in a 37 5 ¡ CO2 condition
Summary
MATERIALS AND METHODSTrivalent chromium has been recognized as a cofactor of insulin[1,2], and has played a prominent physiological role in humans[3,4] and domestic animals[58]. Some studies have indicated that a 200-400ppb trivalent organic chromium supplement can reduce the blood glucose, cholesterol and lipid of mammalian animals, and can reduce bodyfat and increase muscle percentage[3,4,6,8,13,16,]. The experiment in this study was conducted in vitro to determine the effects of supplementation with chromium picolimate on the proliferation and differentiation of myoblasts.
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