Chromatographic separation of saccharide mixtures on zeolites

Abstract

The separation of glucose and fructose by preparative scale liquid chromatography has been accomplished on granulated pellets of a hydrophobic, dealuminated Y-zeolite without Ca++ ion complexation. The separation efficiency is similar to that of a hydrophilic Ca++ exchanged Y-zeolite and also comparable with the common separation on Ca++ loaded ion-exchange resins. In contrast to the hydrophilic zeolites the hydrophobic ones are shown to separate disaccharides. The isolation of isomaltose from a mixture of isomalto-oligosaccharides, and the isolation of inulobiose from fructo-oligosaccharides are presented, also the separation of an industrial molasses of the crystallization of isomaltulose. The addition of small amounts of ethanol to the aqueous eluent not only shortens the retention time but results in more favorable sharpness of the peaks.