Abstract
Abstract Chromatographic methods developed by Paul and Shannon and co-workers for separating individual isozymes of horseradish peroxidase (HRP) have been verified for highly purified but heterogeneous commercial preparations of the enzyme. Chromatography on carboxymethyl cellulose (pH 4·4) reveals that both the Worthington and Calbiochem purification procedures remove the more acidic HRP isozymes present in extracts of horseradish roots.
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