Chromatographic Separation of Heavy Metal Cations on a Silica Gel-G with Amino Acid

S M Wanjari,L J Paliwal,Lata Deshmukh

doi:10.1155/2012/826028

S M Wanjari, L J Paliwal + Show 1 more

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https://doi.org/10.1155/2012/826028

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Abstract

Thin layer chromatographic method has been developed for the separation of metal ions such as Cr(VI), Cr(III), As(III), Cd(II), Tl(III) and Hg(II) from their two, three and four component mixtures. The separations were performed on thin layer of silica gel ‘G’ using aqueous l-Alanine as mobile phase. The effect of concentration and pH of mobile phase on the Rfvalues of individual metal ions were studied and the optimum conditions for separation of metal ions from their mixture were determined.

Highlights

Thin layer chromatography (TLC) is considered to be superior to other chromatographic techniques, because of its simplicity and relatively low cost
TLC has been successfully utilized for various purposes, such as the separation of metal ions from a water sample[1], the characterization of the mobility of metal[2], and the estimation of concentration of toxic metal in industrial waste[3].The use of aqueous solution as a mobile phase in TLC was pioneered by Armstrong and Terrill[4]
The results dealing with the effect of concentration of mobile phase, i.e. on the Rf values of different metal ions such as Cr(VI); Cr(III); As(III); Cd(II); Tl(III) and Hg(II) are represented graphically in fig.1 and tabulated in table 1

Summary

Introduction

Thin layer chromatography (TLC) is considered to be superior to other chromatographic techniques, because of its simplicity and relatively low cost. The use of silica gel and an alumina layer with surfactant mediated mobile phase systems[915] has been used to separate various inorganic species. In this paper chromatographic separation was carried out on silica gel–G using aqueous solution of l-Alanine as mobile phase. Metal ion Studied: Cr(VI); Cr(III); Cd(II); Tl(III); As(III), and Hg(II).

Results

Conclusion