Abstract
Fluoxetine hydrochloride enantiomers were separated by chromatography on Tris‐(3,5‐dimethylphenyl carbamate) cellulose as a chiral stationary phase (CSP). The effects of mobile phase composition, flow rate, and column temperature on capacity factor, separation factor, and resolution were studied systematically. Best separation was obtained with a mobile phase composition of hexane/isopropyl alcohol/diethylamine of 98/2/0.2 (v/v/v) at 15°C. The optimal flow rate was 0.24 mL/min from the Van Deemter equation. However, baseline resolution (RS>1.5) was achieved under a flow rate of 0.8 mL/min. Thermodynamic parameters ΔH 0, Δ S,R ΔH 0, and Δ S,R ΔS 0 were calculated. In the temperature range examined, the enthalpic contribution to the enantiomer transfer energy was found to be more significant than the entropic one.
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