Chromatographic Fingerprinting of the Old World Lupins Seed Alkaloids: A Supplemental Tool in Species Discrimination.

Magdalena Kroc,Wojciech Święcicki,Katarzyna Czepiel,Zygmunt Kaczmarek,Paweł Barzyk,Paulina Wilczura

doi:10.3390/plants8120548

Magdalena Kroc, Wojciech Święcicki + Show 4 more

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https://doi.org/10.3390/plants8120548

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Abstract

The total contents and qualitative compositions of alkaloids in seeds of 10 Old World lupin species (73 accessions) were surveyed using gas chromatography. The obtained results, combined with those for three lupin crops, Lupinus angustifolius, Lupinus albus, and Lupinus luteus, provide the most complete and up-to-date overview of alkaloid profiles of 13 lupin species originating from the Mediterranean Basin. The qualitative alkaloid compositions served as useful supplementary tools of species discrimination. On the basis of the most abundant major alkaloids, lupanine, lupinine, and multiflorine, the Old World lupin species were divided into four groups. Those containing lupanine (L. angustifolius, L. albus, and Lupinus mariae-josephi), containing lupinine (Lupinus luteus, Lupinus hispanicus, and Lupinus × hispanicoluteus), containing lupinine and multiflorine (Lupinus atlanticus, Lupinus palaestinus, Lupinus anatolicus, Lupinus digitatus, Lupinus pilosus, and Lupinus cosentinii), and containing multiflorine (Lupinus micranthus). Within a given group, certain species can be, in most cases, further distinguished by the presence of other major alkaloids. The discrimination of species based on the total alkaloid content was found to be less reliable because of the significant intra-species variations, as well as the influences of environmental factors on the seed alkaloid content.

Highlights

The genus Lupinus consists of two geographically isolated groups of species: the Old World lupins (OWL) and the New World lupins [1]
These results showed again that the qualitative alkaloids. These results showed again that the qualitative alkaloids compositions of L. albus, L. angustifolius, of L.were albus,clearly and L
We presented a joint evaluation of the alkaloid profiles in the seeds of 13 OWL species, encompassing the recently discovered L. anatolicus, L. mariae-josephi, and L. × hispanicoluteus, and in most cases, incorporating several accessions within the species

Summary

Introduction

The genus Lupinus consists of two geographically isolated groups of species: the Old World lupins (OWL) and the New World lupins [1]. A majority of the species within the genus belong to the New. World group, which is distributed in both North and South America [2,3]. In the Mediterranean Basin and North Africa, 12 OWL species have been described, including three lupin crops, Lupinus albus L., Lupinus angustifolius L., and Lupinus luteus L. A Latin diagnosis of three new OWL species, Lupinus anatolicus Swiec. [5], the interspecific hybrid Lupinus × hispanicoluteus Swiec. Two sections related to seed coat texture have been distinguished among the OWL: the smooth-seeded species

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