Abstract
Sarasvata churna is an Ayurvedic formulation prescribed for Epilepsy and other brain related disorders. The objective of this study is to explore bioactive principles present in Sarasvata churna using GCMS and HPTLC techniques and establishing their correlation in Epilepsy treatment. The volatile oil obtained by hydro-distillation was used for the GCMS analysis and methanolic extracts were used for HPTLC studies. HPTLC fingerprints for the formulation and method for simultaneous qualitative analysis of biomarkers in the formulation was developed using HPTLC. The GCMS report shows the presence of α-Pinene, Propanal, 2-Caren-10-al, 3-Caren-10-al, Caryophyllene, Epiglobulol, Asarones, α-selinene, α-sesquiphellandrene, o-cymene, copaene etc. HPTLC fingerprints for methanolic extracts of Sarasvata churna was established with good separation and resolution in solvent system Toluene:Ethylacetate:Methanol (7.5:2.5:0.5v/v/v). The HPTLC method for simultaneous analysis of marker compounds in the formulation was developed in solvent system Ethylacetate:Glycial acetic acid:Toluene:Water (4:1:1:0.5v/v/v/v) with Rf 0.77 for Piperine, 0.07 for Bacosides and Rf 0.57 for Withnolides in the reference compounds and methanolic extracts of Sarasvata churna. Chromatographic patterns of reference compounds were easily detectable in the tracks of Sarasvata churna methanolic extracts. The chromatographic standards obtained from this study will help in chemical standardization of Sarasvata churna and enhance the use Sarasvata churna for management of Epilepsy.
Highlights
Polyherbal formulations are being prescribed for the management of illnesses for thousands of years
The objective of this study is to explore bioactive principles present in Sarasvata churna using GCMS and HPTLC techniques and establishing their correlation in Epilepsy treatment
The HPTLC method for simultaneous analysis of marker compounds in the formulation was developed in solvent system Ethylacetate:Glycial acetic acid:Toluene:Water (4:1:1:0.5 v/v/v/v) with Rf 0.77 for Piperine, 0.07 for Bacosides and Rf 0.57 for Withnolides in the reference compounds and methanolic extracts of Sarasvata churna
Summary
Polyherbal formulations are being prescribed for the management of illnesses for thousands of years. Traditional systems of medicines like Ayurveda, Unani, Siddha, and Homeopathy contain a large number of medicines which contains herbs for prevention and treatment of a variety of diseases. A large pool of human population on earth uses these traditional systems of medicines because of their time-tested efficacy, easy availability, low cost, holistic beliefs and fewer side effects. Ayurvedic text Sarangdhar Samhita emphasizes the use of Polyherbalism in the long-term management of diseases (Kaushik et al, 2017a). Polyherbal formulations contain a large number of bioactive principles contributed by the ingredient herbs in very low concentrations which makes the formulation to act on a disorder by multiple mechanisms of actions along with low toxicity and least side effects. Maximum therapeutic benefits can be achieved only when these formulations are prepared using authentic herbs and used as per the indications mentioned in the ancient texts
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