Abstract
The basic unit of chromatin is nucleosome which is composed of a section of DNA wrapped around a histone octamer. The spacing of nucleosomes defines the degrees of packing of chromatin. The organization of chromatin and its dynamics play crucial roles in harmonizing cell functions. Although highly desired, quantitatively measuring the level of chromatin compaction remains challenging. Previous studies demonstrated an approach, the FRET (Förster resonance energy transfer)-based fluorescence lifetime imaging microscopy (FLIM), to evaluate chromatin compaction.
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