Chondrogenic capacity of CD105-positive synovium-derived mesenchymal stem cells

Abstract

Objective To evaluate the proliferation and chondrogenic differentiation of CD105+ enriched synovium-derived mesenchymal stem cells(SMSCs). Methods The synovium-derived mesenchymal stem cells(SMSCs)were obtained from human synovium by enzyme digestion and CD105- positive(CD105+)cells were enriched using fluorescence activated cell sorting(FACS). The proliferation ability of CD105+ SMSCs was measured through WST-1 at day 3 and day 7.The chondrogenic differentiation of CD105+ SMSCs was detected by toluidine blue staining for aggrecan and immunohistochemistry staining for typeⅡcollagen after 21 days introduction. Results The synovium-derived mesenchymal stem cells(SMSCs) showed star or spindle shape. No obvious differences in morphology were noted after sorting. Immunofluorescence staining showed that there were more CD105+ cells in the sorted group than the unsorted. There was statistical difference between sorted and unsorted cells at day 3(0.376±0.012,0.329±0.012)and day 7(0.581±0.009,0.524± 0.007)on the A value by WST-1(P< 0.05). The toluidine blue staining for aggrecan and immunohistochemistry staining for type Ⅱ collagen were more and stronger in sorted cells than unsorted after 21 days chondrogenic differentiation, which indicated that CD105+ SMSCs synthesized more cartilage extracellular matrix. Conclusion The CD105+ SMSCs have stronger proliferation ability and chondrogenic capacity, which may be promising cell sources for cartilage tissue engineering. Key words: Synovium-derived mesenchymal stem cells; CD105; Cartilage tissue engineering