The experimental study on migration of synovium-derived mesenchymal stem cells by five chemotactic factors

Abstract

Objective To explore the chemotactic migration effects of serum,transforming growth factor (TGF)-β3,platelet derived growtb factor (PDGF)-AB,bone morphogenetic protein (BMP)-2,and fibroblast growth factor (FGF) on rabbit synovium-derived mesenchymal stem cells (SMSCs).Methods The SMSCs were obtained with passage isolation,and then stimulated bv low-glucose DEME containing TGF-β3,PDGF-AB,BMP-2,FGF with 50 μg/L concentration as well as 0.1％,0.5％,1.0％,2.0％,5.0％,10.0％,20.0％ serum.The migration of SMSCs under five different chemotactic factors was observed in Transwell chamber assays.Results TGF-β3,PDGF-AB,BMP-2,FGF and serum consistently enhanced the migration activity of rabbit SMSCs.Compared witb other concentration,5.0％ is the appropriate concentration for serum to induce the migration of rabbit SMSCs.The average numbers of migratory cells (SMSCs) at × 100 magnification was 169.00 ±21.74 in lower chamber under the 5.0％ serum within the Transwell assay.Among the four growth factors,PDGF-AB showed more potent chemotactic effect than otherthree (219.83±11.29 vs.84.50±10.33,146.67±17.91,99.37±12.27).Conclusion The migration of SMSCs from rabbit could be enhanced by TGF-β3,PDGF-AB,BMP-2 and FGF.PDGF-AB is the most potent chemotactic factor among the four growth factors,and may be useful to promote cell homing and cartilage regeneration in situ during cartilage repair. Key words: Synovium-derived mesenchymal stem cells; Cytokine; Chemotaxis; Migration