Abstract
Abstract An automated system encompassing stopped-flow techniques and automated data acquisition and processing has been developed to conduct studies on cholinesterase kinetics, including inhibition experiments for the evaluation of the binding constant, K d , the first-order inhibition constant, k 2 , and the overall second-order inhibition constant, k i . The instrumentation, hardware, and software have been developed and combined such that no training in computer languages and only a limited knowledge of computer systems interaction is necessary for its use. Application of the system to kinetic investigations requires no manual data gathering and manipulation. The automated system has been utilized in determining the inhibition constants for a combination of three inhibitors, paraoxon, amiton, and carbaryl, and two acetylcholinesterases, bovine erythrocyte and eel, obtaining six sets of inhibition constants.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
