Abstract
By the use of centrifugal fractionation, specific substrates and selective inhibitors evidence was obtained that the diaphragm muscle of guinea pig, man and rat contain both soluble and particulate acetylcholinesterase (EC 3.1.1.7) and a soluble cholinesterase (EC 3.1.1.8). This is believed to be the first report of naturally-occurring soluble mammalian acetylcholinesterases. Human and rat diaphragms also contain a physostigmine-resistant enzyme which hydrolyses butyrylcholine, and is probably a B-esterase. It was noted incidentally that a modified Koelle histochemical method, using thiocholine esters, has a specific requirement for iodide ion which has not previously been reported.
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