Choline acetyltransferase expression during a putative developmental waiting period

Abstract

The relationship between the cholinergic expression, morphological development, and target cell innervation of olivocochlear (OC) efferent neurons was investigated in the postnatal hamster. Similar to what was found in previous studies, tracer injections into the contralateral cochlea labeled cells bodies retrogradely in periolivary regions and labeled cell bodies only rarely in the lateral superior olive (LSO). Few morphological differences were found among cell bodies labeled between postnatal day 1 (P1) and P30. Tracer injections into the crossed OC bundles within the brainstem anterogradely labeled terminals below the inner hair cells of the cochlea prior to P5 and labeled terminals below outer hair cells after P5, consistent with a period of transient innervation, as hypothesized previously. Within the superior olive, choline acetyltransferase (ChAT) was expressed differentially. In periolivary regions, ChAT was expressed as early as P0. ChAT-immunoreactive cell bodies in periolivary regions were similar morphologically to retrogradely labeled OC neurons. In contrast, within the LSO, ChAT was not expressed until after P2. Consistent with a medical OC projection to the cochlea at early postnatal ages, ChAT immunoreactivity was detected below inner hair cells as early as P2 but was not detected below outer hair cells until after P6. Our results suggest that medial OC neurons not only provide transient connections to inner hair cells but also may express ChAT when they are below inner hair cells. Furthermore, these results raise the possibility that OC neurons may be capable of acetylcholine synthesis and release prior to or simultaneous with their innervation of the cochlea.