Abstract
A technique for chloroplast isolation from the brown algae macrophyte Laminaria digitata (Phaeophyceae) is described which results in stable and photo-synthetically active chloroplasts. Chloroplasts in L. digitata are mainly located in the surface layer meristodermic cells. The expanded intercellular regions of the cortex tissue contain abundant mucilage which is exuded during mechanical tissue disruption. The method described for chloroplast isolation is based on appropriate isolation buffers and the removal of mucilage which is secreted during tissue disruption, and it may be applicable to other macroalgae with large amounts of mucilage. The isolated chloroplasts showed stable oxygen-evolving activities. The reproducibility of this technique was established by analysis of electron transport and evaluation of the activities of photosystems I and II.
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