Abstract
Simultaneous equations for the determination of monovinyl (MV) and divinyl (DV) Chl a and b by spectrofluorometry in unsegregated mixtures of these tetrapyrroles were derived. The same equations can also be used for the quantitative determination of MV and DV chlorophyllide (Chlide) a and b in unpurified mixtures, after extraction of the Chls in hexane. The equations used differences in the Soret excitation maxima of these tetrapyrroles in ether at 77°K, in order to correct the Soret excitation overlap between MV and DV Chl(ide) a and between MV and DV Chl(ide) b.
Full Text
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call